CATH Classification

Domain Context

CATH Clusters

Superfamily Neutral Protease Domain 2
Functional Family Thermolysin

Enzyme Information

3.4.24.27
Thermolysin.
based on mapping to UniProt P00800
Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.
-!- A thermostable extracellular metalloendopeptidase containing four calcium ions. -!- Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus and Aspergillus oryzae. -!- Closely related but distinct enzymes are aeromonolysin, pseudolysin, bacillolysin, aureolysin and mycolysin. -!- Belongs to peptidase family M4. -!- Formerly EC 3.4.24.4.

UniProtKB Entries (1)

P00800
THER_BACTH
Bacillus thermoproteolyticus
Thermolysin

PDB Structure

PDB 4TLN
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis.
Holmes, M.A., Matthews, B.W.
Biochemistry