CATH Classification

Domain Context

CATH Clusters

Functional Family 5-hydroxymethyluracil DNA glycosylase

Enzyme Information
DNA-formamidopyrimidine glycosylase.
based on mapping to UniProt P42371
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
-!- May play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents. -!- Also involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA.
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P42371
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC

UniProtKB Entries (1)

Lactococcus lactis subsp. cremoris
Formamidopyrimidine-DNA glycosylase

PDB Structure

External Links
Primary Citation
Zinc finger oxidation of Fpg/Nei DNA glycosylases by 2-thioxanthine: biochemical and X-ray structural characterization.
Biela, A., Coste, F., Culard, F., Guerin, M., Goffinont, S., Gasteiger, K., Ciesla, J., Winczura, A., Kazimierczuk, Z., Gasparutto, D., Carell, T., Tudek, B., Castaing, B.
Nucleic Acids Res.