CATH Classification

Domain Context

CATH Clusters

Superfamily Nucleoside Triphosphate Pyrophosphohydrolase
Functional Family mRNA-decapping enzyme 2

Enzyme Information

3.6.1.62
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase.
based on mapping to UniProt P53550
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] + H(2)O = N(7)- methylguanosine 5'-diphosphate + 5'-phospho-[mRNA].
-!- Decapping of mRNA is a critical step in eukaryotic mRNA turnover. -!- The enzyme is unable to cleave a free cap structure (m(7)GpppG). -!- The enzyme from Vaccinia virus is synergistically activated in the presence of Mg(2+) and Mn(2+). -!- Formerly EC 3.6.1.30.

UniProtKB Entries (1)

P53550
DCP2_YEAST
Saccharomyces cerevisiae S288C
M7GpppN-mRNA hydrolase

PDB Structure

PDB 4KG4
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Active site conformational dynamics are coupled to catalysis in the mRNA decapping enzyme dcp2.
Aglietti, R.A., Floor, S.N., McClendon, C.L., Jacobson, M.P., Gross, J.D.
Structure