CATH Classification

Domain Context

CATH Clusters

Superfamily 2.40.50.830
Functional Family mRNA-capping enzyme catalytic subunit

Enzyme Information

2.7.7.50
mRNA guanylyltransferase.
based on mapping to UniProt P04298
GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
-!- Can modify also synthetic poly(A) and poly(G) to form the structures m(7)G(5')pppAn and m(7)G(5')pppGn.
3.1.3.33
Polynucleotide 5'-phosphatase.
based on mapping to UniProt P04298
A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.
-!- Does not act on nucleoside monophosphates. -!- Induced in Escherichia coli by T-even phages.
2.1.1.56
mRNA (guanine-N(7)-)-methyltransferase.
based on mapping to UniProt P04298
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
-!- Adds an N(7)-methylguanine cap to mRNA. -!- The nucleoside next to the terminal guanosine may be either guanosine or adenosine.

UniProtKB Entries (1)

P04298
MCEL_VACCW
Vaccinia virus WR
MRNA-capping enzyme catalytic subunit

PDB Structure

PDB 4CKE
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal Structure of Vaccinia Virus Mrna Capping Enzyme Provides Insights Into the Mechanism and Evolution of the Capping Apparatus.
Kyrieleis, O.J.P., Chang, J., De La Pena, M., Shuman, S., Cusack, S.
Structure