CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.510 | Transferase(Phosphotransferase); domain 1 |
|
1.10.510.10 | Transferase(Phosphotransferase) domain 1 |
Domain Context
CATH Clusters
| Superfamily | Transferase(Phosphotransferase) domain 1 |
| Functional Family |
Enzyme Information
| 2.7.1.181 |
Polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol kinase.
based on mapping to UniProt Q47592
ATP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-(alpha-D-Man-(1->3)-alpha-D- Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2))(n)-alpha-D-Man-(1->3)- alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho- ditrans,octacis-undecaprenol = ADP + 3-O-phospho-alpha-D-Man-(1->2)- alpha-D-Man-(1->2)-(alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man- (1->2)-alpha-D-Man-(1->2))(n)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)- alpha-D-Man-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol.
-!- The enzyme is involved in the biosynthesis of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotype O9a. -!- O-polysaccharide structures vary extensively because of differences in the number and type of sugars in the repeat unit. -!- The dual kinase/methylase WbdD also catalyzes the methylation of 3-phospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-(alpha-D-Man-(1->3)- alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2))(n)-alpha-D- Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-GlcNAc- diphospho-ditrans,octacis-undecaprenol (Cf. EC 2.1.1.294).
|
| 2.1.1.294 |
3-O-phospho-polymannosyl GlcNAc-diphospho-ditrans,octacis-undecaprenol 3-phospho-methyltransferase.
based on mapping to UniProt Q47592
S-adenosyl-L-methionine + 3-O-phospho-alpha-D-Man-(1->2)-alpha-D-Man- (1->2)-(alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D- Man-(1->2))(N)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)- alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol = S-adenosyl-L- homocysteine + 3-O-methylphospho-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)- (alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man- (1->2))(N)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)- alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol.
-!- The enzyme is involved in the biosynthesis of the polymannose O-polysaccharide in the outer leaflet of the membrane of Escherichia coli serotype O9a. -!- O-polysaccharide structures vary extensively because of differences in the number and type of sugars in the repeat unit. -!- The dual kinase/methylase WbdD also catalyzes the preceding phosphorylation of alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-(alpha-D- Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man- (1->2))(N)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)-alpha-D-Man-(1->3)- alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol (cf. EC 2.7.1.181).
|
UniProtKB Entries (1)
| Q47592 |
WBDD2_ECOLX
Escherichia coli
O-antigen chain terminator bifunctional methyltransferase/kinase WbdD
|
PDB Structure
| PDB | 4AZW |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structure of Wbdd; a Bifunctional Kinase and Methyltransferase that Regulates the Chain Length of the O Antigen in Escherichia Coli O9A.
Mol.Microbiol.
|