CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.11270
Functional Family 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Enzyme Information

1.17.7.4
4-hydroxy-3-methylbut-2-enyl diphosphate reductase.
based on mapping to UniProt P62623
(1) Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+). (2) Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+).
-!- Forms a system with a ferredoxin or a flavodoxin and an NAD(P)H-dependent reductase. -!- This is the last enzyme in the non-mevalonate pathway for isoprenoid biosynthesis. -!- This pathway, also known as the 1-deoxy-D-xylulose 5-phosphate (DOXP) or as the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, is found in most bacteria and in plant chloroplasts. -!- The enzyme acts in the reverse direction, producing a 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate. -!- Formerly EC 1.17.1.2.

UniProtKB Entries (1)

P62623
ISPH_ECOLI
Escherichia coli K-12
4-hydroxy-3-methylbut-2-enyl diphosphate reductase

PDB Structure

PDB 3SZO
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal Structures of Mutant IspH Proteins Reveal a Rotation of the Substrate's Hydroxymethyl Group during Catalysis.
Span, I., Grawert, T., Bacher, A., Eisenreich, W., Groll, M.
J.Mol.Biol.