CATH Classification

Domain Context

CATH Clusters

Superfamily 2.20.25.160
Functional Family Aspartoacylase

Enzyme Information

3.5.1.114
N-acyl-aromatic-L-amino acid amidohydrolase.
based on mapping to UniProt Q91XE4
(1) An N-acyl-aromatic-L-amino acid + H(2)O = an aromatic-L-amino acid + a carboxylate. (2) An N-acetyl-L-cysteine-S-conjugate + H(2)O = an L-cysteine-S- conjugate + acetate.
-!- This enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). -!- It preferentially deacetylates N(alpha)-acetylated aromatic amino acids and mercapturic acids (S-conjugates of N-acetyl-L-cysteine) that are usually not deacetylated by EC 3.5.1.14. -!- Some bacterial aminoacylases demonstrate substrate specificity for both EC 3.5.1.14 and EC 3.5.1.114. -!- Cf. EC 3.5.1.14 and EC 3.5.1.15.

UniProtKB Entries (1)

Q91XE4
ACY3_MOUSE
Mus musculus
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)

PDB Structure

PDB 3NH5
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structures of aminoacylase 3 in complex with acetylated substrates.
Hsieh, J.M., Tsirulnikov, K., Sawaya, M.R., Magilnick, N., Abuladze, N., Kurtz, I., Abramson, J., Pushkin, A.
Proc.Natl.Acad.Sci.USA