CATH Classification

Domain Context

CATH Clusters

Superfamily Radical SAM ThiC family, central domain
Functional Family Phosphomethylpyrimidine synthase

Enzyme Information

4.1.99.17
Phosphomethylpyrimidine synthase.
based on mapping to UniProt Q9A6Q5
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphomethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.
-!- Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. -!- The first stage of catalysis is reduction of the S-adenosyl-L- methionine to produce L-methionine and a 5'-deoxyadenosin-5'-yl radical that is crucial for the conversion of the substrate. -!- Part of the pathway for thiamine biosynthesis.

UniProtKB Entries (1)

Q9A6Q5
THIC_CAUVC
Caulobacter vibrioides CB15
Phosphomethylpyrimidine synthase

PDB Structure

PDB 3EPN
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily
Chatterjee, A., Li, Y., Zhang, Y., Grove, T.L., Lee, M., Krebs, C., Booker, S.J., Begley, T.P., Ealick, S.E.
Nat.Chem.Biol.