CATH Classification

Domain Context

CATH Clusters

Superfamily Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2
Functional Family

Enzyme Information

1.5.99.12
Cytokinin dehydrogenase.
based on mapping to UniProt Q9T0N8
N(6)-dimethylallyladenine + acceptor + H(2)O = adenine + 3-methylbut-2- enal + reduced acceptor.
-!- Converts zeatin and related cytokinins. -!- Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group. -!- Although this activity was previously thought to be catalyzed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide. -!- 2,6-dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(2+) in the presence of imidazole can act as acceptors.

UniProtKB Entries (1)

Q9T0N8
CKX1_MAIZE
Zea mays
Cytokinin dehydrogenase 1

PDB Structure

PDB 3DQ0
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Characterization and biological activity of novel purine-derived inhibitor of cytokinin oxidase/dehydrogenase and its potential use for in vivo studies
Spichal, L., Werner, T., Galuszka, P., Zatloukal, T., Kopecny, D., Briozzo, P., Nisler, J., Gruz, J., Frebortova, J., Schmulling, T., Strnad, M.
To be Published