CATH Classification

Domain Context

CATH Clusters

Superfamily C2 domain
Functional Family Putative E3 ubiquitin-protein ligase NEDD4-like

Enzyme Information

2.3.2.26
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt P46934
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

P46934
NEDD4_HUMAN
Homo sapiens
E3 ubiquitin-protein ligase NEDD4

PDB Structure

PDB 3B7Y
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
C2 Domain of the Human E3 Ubiquitin-Protein Ligase NEDD4.
Walker, J.R., Ruzanov, M., Butler-Cole, C., Weigelt, J., Arrowsmith, C.H., Edwards, A.M., Bochkarev, A., Dhe-Paganon, S.
To be Published