CATH Classification

Domain Context

CATH Clusters

Superfamily Butyryl-CoA Dehydrogenase, subunit A, domain 2
Functional Family Flavin-dependent monooxygenase, oxygenase subunit

Enzyme Information

1.14.14.12
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase.
based on mapping to UniProt P9WJA1
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O.
-!- This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. -!- It can use either FADH or FMNH(2) as flavin cofactor. -!- The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.

UniProtKB Entries (1)

P9WJA1
HSAA_MYCTU
Mycobacterium tuberculosis H37Rv
Flavin-dependent monooxygenase, oxygenase subunit HsaA

PDB Structure

PDB 3AFF
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism
Dresen, C., Lin, L.Y., D'Angelo, I., Tocheva, E.I., Strynadka, N., Eltis, L.D.
J.Biol.Chem.