CATH Classification

CATH Clusters

Superfamily 3.40.47.10
Functional Family Probable 3-oxoacyl-[acyl-carrier-protein] synthase i

Enzyme Information

1.1.1.100
3-oxoacyl-[acyl-carrier-protein] reductase.
based on mapping to UniProt P19097
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl- carrier-protein] + NADPH.
-!- Exhibits a marked preference for [acyl-carrier-protein] derivatives over CoA derivatives as substrates.
2.3.1.41
Beta-ketoacyl-[acyl-carrier-protein] synthase I.
based on mapping to UniProt P19097
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO(2) + [acyl-carrier-protein].
-!- Responsible for the chain-elongation step of dissociated (type II) fatty-acid biosynthesis, i.e. the addition of two C atoms to the fatty-acid chain. -!- Escherichia coli mutants that lack this enzyme are deficient in unsaturated fatty acids. -!- Can use fatty acyl thioesters of ACP (C(2) to C(16)) as substrates, as well as fatty acyl thioesters of Co-A (C(4) to C(16)). -!- The substrate specificity is very similar to that of EC 2.3.1.179 with the exception that the latter enzyme is far more active with palmitoleoyl-ACP (C(16)-Delta(9)) as substrate, allowing the organism to regulate its fatty-acid composition with changes in temperature.
2.3.1.86
Fatty-acyl-CoA synthase.
based on mapping to UniProt P19097
Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+).
-!- The enzyme from yeasts (Ascomycota and Basidiomycota) is a multi- functional protein complex composed of two subunits. -!- One subunit catalyzes the reactions EC 1.1.1.100 and EC 2.3.1.41, while the other subunit catalyzes the reactions of EC 2.3.1.38, EC 2.3.1.39, EC 4.2.1.59, EC 1.3.1.10 and EC 1.1.1.279. -!- The enzyme differs from the animal enzyme (EC 2.3.1.85) in that the enoyl reductase domain requires FMN as a cofactor, and the ultimate product is an acyl-CoA (usually palmitoyl-CoA) instead of a free fatty acid.

UniProtKB Entries (1)

P07149
FAS1_YEAST
Saccharomyces cerevisiae S288C
Fatty acid synthase subunit beta

PDB Structure

PDB 2UV8
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural Basis for Substrate Delivery by Acyl Carrier Protein in the Yeast Fatty Acid Synthase
Leibundgut, M., Jenni, S., Frick, C., Ban, N.
Science