CATH Classification

Domain Context

CATH Clusters

Superfamily Bira Bifunctional Protein; Domain 2
Functional Family

Enzyme Information

2.3.1.181
Lipoyl(octanoyl) transferase.
based on mapping to UniProt Q5SLQ3
Octanoyl-[acyl-carrier-protein] + protein = protein N(6)-(octanoyl)lysine + [acyl-carrier-protein].
-!- The first committed step in the biosynthesis of lipoyl cofactor. -!- The lipoyl cofactor is essential for the function of several key enzymes involved in oxidative metabolism, as it converts apoprotein into the biologically active holoprotein. -!- Examples of such lipoylated proteins include pyruvate dehydrogenase (E(2) domain), 2-oxoglutarate dehydrogenase (E(2) domain), the branched-chain 2-oxoacid dehydrogenases and the glycine cleavage system (H protein). -!- Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the true substrate. -!- The other enzyme involved in the biosynthesis of lipoyl cofactor is EC 2.8.1.8. -!- An alternative lipoylation pathway involves EC 2.7.7.63, which can lipoylate apoproteins using exogenous lipoic acid (or its analogs).

UniProtKB Entries (1)

Q5SLQ3
LIPB_THET8
Thermus thermophilus HB8
Octanoyltransferase

PDB Structure

PDB 2QHS
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural basis of octanoic acid recognition by lipoate-protein ligase B
Kim, D.J., Lee, S.J., Kim, H.S., Kim, K.H., Lee, H.H., Yoon, H.J., Suh, S.W.
Proteins