CATH Classification

Domain Context

CATH Clusters

Superfamily DNA helicase RuvA subunit, C-terminal domain
Functional Family Putative E3 ubiquitin-protein ligase UBR5

Enzyme Information

2.3.2.26
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt O95071
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (2)

P0CH28
UBC_BOVIN
Bos taurus
Polyubiquitin-C
O95071
UBR5_HUMAN
Homo sapiens
E3 ubiquitin-protein ligase UBR5

PDB Structure

PDB 2QHO
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural basis of ubiquitin recognition by the ubiquitin-associated (UBA) domain of the ubiquitin ligase EDD.
Kozlov, G., Nguyen, L., Lin, T., De Crescenzo, G., Park, M., Gehring, K.
J.Biol.Chem.