CATH Classification

Domain Context

CATH Clusters

Superfamily PUA domain
Functional Family Probable tRNA pseudouridine synthase B

Enzyme Information

5.4.99.25
tRNA pseudouridine(55) synthase.
based on mapping to UniProt Q7LWY0
tRNA uridine(55) = tRNA pseudouridine(55).
-!- Pseudourine synthase TruB from Escherichia coli specifically modifies uridine(55) in tRNA molecules. -!- The bifunctional archaeal enzyme also catalyzes the pseudouridylation of uridine(54). -!- It is not known whether the enzyme from Escherichia coli can also act on position 54 in vitro, since this position is occupied in E.coli tRNAs by thymine.

UniProtKB Entries (1)

Q8U1R4
NOP10_PYRFU
Pyrococcus furiosus DSM 3638
Ribosome biogenesis protein Nop10

PDB Structure

PDB 2EY4
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita.
Rashid, R., Liang, B., Baker, D.L., Youssef, O.A., He, Y., Phipps, K., Terns, R.M., Terns, M.P., Li, H.
Mol.Cell