CATH Classification

Domain Context

CATH Clusters

Superfamily Aspartate Aminotransferase, domain 1
Functional Family

Enzyme Information

2.5.1.73
O-phospho-L-seryl-tRNA:Cys-tRNA synthase.
based on mapping to UniProt O30207
O-phospho-L-seryl-tRNA(Cys) + sulfide = L-cysteinyl-tRNA(Cys) + phosphate.
-!- In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 for the direct Cys-tRNA(Cys) formation, Cys-tRNA(Cys) is produced by an indirect pathway, in which EC 6.1.1.27 ligates O-phosphoserine to tRNA(Cys), and EC 2.5.1.73 converts the produced O-phospho-L-seryl- tRNA(Cys) to Cys-tRNA(Cys). -!- The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism. -!- Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73. -!- Formerly EC 2.5.1.n3.

UniProtKB Entries (1)

O30207
SPSS1_ARCFU
Archaeoglobus fulgidus DSM 4304
O-phospho-L-seryl-tRNA:Cys-tRNA synthase 1

PDB Structure

PDB 2E7J
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural Insights into the Second Step of RNA-dependent Cysteine Biosynthesis in Archaea: Crystal Structure of Sep-tRNA:Cys-tRNA Synthase from Archaeoglobus fulgidus
Fukunaga, R., Yokoyama, S.
J.Mol.Biol.