CATH Classification

Domain Context

CATH Clusters

Superfamily Cysteine proteinases
Functional Family Aminopeptidase C

Enzyme Information

3.4.22.40
Bleomycin hydrolase.
based on mapping to UniProt Q01532
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.
-!- The active sites are on the walls of a central channel through the molecule, and access of substrate molecules to them is obstructed by this and by the C-terminus of each polypeptide chain. -!- Bleomycin can scarcely be the natural substrate, and there are reports of limited endopeptidase activity. -!- Known from bacteria as well as eukaryotic organisms. -!- Hydrolase H from chicken muscle has many similarities to bleomycin hydrolase, but hydrolyzes Ph-CO-Arg-2-naphthylamine as well as aminopeptidase substrates. -!- Belongs to peptidase family C1.

UniProtKB Entries (1)

Q01532
BLH1_YEAST
Saccharomyces cerevisiae S288C
Cysteine proteinase 1, mitochondrial

PDB Structure

PDB 2E00
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Mutagenesis and crystallographic studies of the catalytic residues of the papain family protease bleomycin hydrolase: new insights into active-site structure
O'Farrell, P.A., Joshua-Tor, L.
Biochem.J.