CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family Thiazole biosynthesis adenylyltransferase ThiF

Enzyme Information

2.7.7.73
Sulfur carrier protein ThiS adenylyltransferase.
based on mapping to UniProt P30138
ATP + [ThiS] = diphosphate + adenylyl-[ThiS].
-!- The enzyme catalyzes the adenylation of ThiS, a sulfur carrier protein involved in the biosynthesis of thiamine. -!- The enzyme shows significant structural similarity to ubiquitin- activating enzyme. -!- In Escherichia coli, but not in Bacillus subtilis, the enzyme forms a cross link from Cys-184 to the ThiS carboxy terminus (the position that is also thiolated) via an acyldisulfide. -!- Formerly EC 2.7.7.n3.

UniProtKB Entries (1)

O32583
THIS_ECOLI
Escherichia coli K-12
Sulfur carrier protein ThiS

PDB Structure

PDB 1ZUD
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of the Escherichia coli ThiS-ThiF Complex, a Key Component of the Sulfur Transfer System in Thiamin Biosynthesis.
Lehmann, C., Begley, T.P., Ealick, S.E.
Biochemistry