CATH Classification

Domain Context

CATH Clusters

Superfamily 1.10.720.60
Functional Family Enolase-phosphatase E1

Enzyme Information

3.1.3.77
Acireductone synthase.
based on mapping to UniProt Q9UHY7
5-(methylthio)-2,3-dioxopentyl phosphate + H(2)O = 1,2-dihydroxy-5- (methylthio)pent-1-en-3-one + phosphate.
-!- This bifunctional enzyme first enolizes the substrate to form the intermediate 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2- dihydroxy-5-(methylsulfanyl)pent-1-en-3-one. -!- The acireductone represents a branch point in the methione salvage pathway as it is used in the formation of formate, CO and 3-(methylsulfanyl)propanoate by EC 1.13.11.53 and of formate and 4-(methylsulfanyl)-2-oxobutanoate either by a spontaneous reaction under aerobic conditions or by EC 1.13.11.54.

UniProtKB Entries (1)

Q9UHY7
ENOPH_HUMAN
Homo sapiens
Enolase-phosphatase E1

PDB Structure

PDB 1ZS9
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal Structure of Human E1 Enzyme and its Complex with a Substrate Analog Reveals the Mechanism of its Phosphatase/Enolase
Wang, H., Pang, H., Bartlam, M., Rao, Z.
J.Mol.Biol.