CATH Classification

Domain Context

CATH Clusters

Superfamily Methyltransferase, Cobalt-precorrin-4 Transmethylase; Domain 2
Functional Family Diphthine synthase putative

Enzyme Information

2.1.1.98
Diphthine synthase.
based on mapping to UniProt O58456
3 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine- [translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2].
-!- This archaeal enzyme produces the trimethylated product diphthine, which is converted into diphthamide by EC 6.3.1.14. -!- Different from the eukaryotic enzyme, which produces diphthine methyl ester (cf. EC 2.1.1.314). -!- In the archaeon Pyrococcus horikoshii the enzyme acts on His(600) of elongation factor 2.

UniProtKB Entries (1)

O58456
DPHB_PYRHO
Pyrococcus horikoshii OT3
Diphthine synthase

PDB Structure

PDB 1WNG
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structures of two archaeal diphthine synthases: insights into the post-translational modification of elongation factor 2.
Kishishita, S., Shimizu, K., Murayama, K., Terada, T., Shirouzu, M., Yokoyama, S., Kunishima, N.
Acta Crystallogr.,Sect.D