CATH Classification

Domain Context

CATH Clusters

Superfamily Pseudouridine synthase
Functional Family tRNA pseudouridine synthase B

Enzyme Information

5.4.99.25
tRNA pseudouridine(55) synthase.
based on mapping to UniProt P9WHP7
tRNA uridine(55) = tRNA pseudouridine(55).
-!- Pseudourine synthase TruB from Escherichia coli specifically modifies uridine(55) in tRNA molecules. -!- The bifunctional archaeal enzyme also catalyzes the pseudouridylation of uridine(54). -!- It is not known whether the enzyme from Escherichia coli can also act on position 54 in vitro, since this position is occupied in E.coli tRNAs by thymine.

UniProtKB Entries (1)

P9WHP7
TRUB_MYCTU
Mycobacterium tuberculosis H37Rv
TRNA pseudouridine synthase B

PDB Structure

PDB 1SGV
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft.
Chaudhuri, B.N., Chan, S., Perry, L.J., Yeates, T.O.
J.Biol.Chem.