CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.1100
Functional Family 1-aminocyclopropane-1-carboxylate deaminase

Enzyme Information

3.5.99.7
1-aminocyclopropane-1-carboxylate deaminase.
based on mapping to UniProt Q00740
1-aminocyclopropane-1-carboxylate + H(2)O = 2-oxobutanoate + NH(3).
-!- The enzyme, found in certain soil bacteria and fungi, catalyzes the ring opening of 1-aminocyclopropane-1-carboxylate, the immediate precursor to ethylene, an important plant hormone that regulates fruit ripening and other processes. -!- The enzyme releases an unstable enamine product that tautomerizes to an imine form, which undergoes spontaneous hydrolytic deamination. -!- The enzyme has been used to make fruit ripening dependent on externally added ethylene, as it removes the substrate for endogenous ethylene formation. -!- Formerly EC 4.1.99.4.

UniProtKB Entries (1)

Q00740
1A1D_PSEUD
Pseudomonas sp. ACP
1-aminocyclopropane-1-carboxylate deaminase

PDB Structure

PDB 1RQX
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural analysis of 1-aminocyclopropane-1-carboxylate deaminase: observation of an aminyl intermediate and identification of Tyr 294 as the active-site nucleophile.
Karthikeyan, S., Zhao, Z., Kao, C.L., Zhou, Q., Tao, Z., Zhang, H., Liu, H.W.
Angew.Chem.Int.Ed.Engl.