CATH Classification

Domain Context

CATH Clusters

Superfamily Ribonuclease T2-like
Functional Family

Enzyme Information

4.6.1.19
Ribonuclease T(2).
based on mapping to UniProt P23540
RNA + H(2)O = an (RNA fragment)-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment).
-!- A widely distributed family of related enzymes found in protozoans, plants, bacteria, animals and viruses that cleave ssRNA 3'-phosphate group with little base specificity. -!- The enzyme catalyzes a two-stage endonucleolytic cleavage. -!- The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending with a 2',3'-cyclic phosphodiester, which are released from the enzyme. -!- The enzyme then hydrolyzes the cyclic products in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. -!- The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides. -!- Formerly EC 2.7.7.17, EC 3.1.4.23, and EC 3.1.27.1.

UniProtKB Entries (1)

P23540
RNMC_MOMCH
Momordica charantia
Ribonuclease MC

PDB Structure

PDB 1J1F
External Links
Method X-RAY DIFFRACTION
Organism Pichia
Primary Citation
Crystal Structures of the Ribonuclease MC1 Mutants N71T and N71S in Complex with 5'-GMP: Structural Basis for Alterations in Substrate Specificity
Numata, T., Suzuki, A., Kakuta, Y., Kimura, K., Yao, M., Tanaka, I., Yoshida, Y., Ueda, T., Kimura, M.
Biochemistry