CATH Classification

Domain Context

CATH Clusters

Superfamily SCP2 sterol-binding domain
Functional Family Hydroxysteroid 17-beta dehydrogenase 4

Enzyme Information

4.2.1.107
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase.
based on mapping to UniProt P51659
(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl- CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl- CoA + H(2)O.
-!- Forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. -!- Involved in the beta-oxidation of the cholesterol side chain in the cholic-acid biosynthesis pathway.
4.2.1.119
Enoyl-CoA hydratase 2.
based on mapping to UniProt P51659
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O.
-!- This enzyme catalyzes a hydration step in peroxisomal beta-oxidation. -!- The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units. -!- The enzymes from Aeromonas caviae and Arabidopsis thaliana are monofunctional enzymes. -!- 2-enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2.
1.1.1.n12
(3R)-hydroxyacyl-CoA dehydrogenase.
based on mapping to UniProt P51659
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.

UniProtKB Entries (1)

P51659
DHB4_HUMAN
Homo sapiens
Peroxisomal multifunctional enzyme type 2

PDB Structure

PDB 1IKT
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of the liganded SCP-2-like domain of human peroxisomal multifunctional enzyme type 2 at 1.75 A resolution.
Haapalainen, A.M., van Aalten, D.M., Merilainen, G., Jalonen, J.E., Pirila, P., Wierenga, R.K., Hiltunen, J.K., Glumoff, T.
J.Mol.Biol.