CATH Classification

Domain Context

CATH Clusters

Superfamily Methylated DNA-protein cysteine methyltransferase domain
Functional Family Methylated-DNA--protein-cysteine methyltransferase

Enzyme Information

2.1.1.63
Methylated-DNA--[protein]-cysteine S-methyltransferase.
based on mapping to UniProt P16455
(1) DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. (2) DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.
-!- This protein is involved in the repair of methylated DNA. -!- Unlike EC 3.2.2.20 and EC 3.2.2.21, which remove the methylated base leaving an apurinic/apyrimidinic site, this enzyme transfers the methyl group from the methylated DNA to an internal cysteine residue, leaving an intact nucleotide. -!- Since the methyl transfer is irreversible, the enzyme can only catalyze a single turnover.

UniProtKB Entries (1)

P16455
MGMT_HUMAN
Homo sapiens
Methylated-DNA--protein-cysteine methyltransferase

PDB Structure

PDB 1EH8
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding.
Daniels, D.S., Mol, C.D., Arvai, A.S., Kanugula, S., Pegg, A.E., Tainer, J.A.
EMBO J.