CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.720 | NAD(P)-binding Rossmann-like Domain |
Domain Context
CATH Clusters
| Superfamily | NAD(P)-binding Rossmann-like Domain |
| Functional Family |
Enzyme Information
| 1.5.1.28 |
Opine dehydrogenase.
based on mapping to UniProt Q44297
(2S)-2-((1-(R)-carboxyethyl)amino)pentanoate + NAD(+) + H(2)O = L-2- aminopentanoic acid + pyruvate + NADH.
-!- In the forward direction, the enzyme from Arthrobacter sp. acts also on secondary amine dicarboxylates such as N-(1-carboxyethyl)methionine and N-(1-carboxyethyl)phenylalanine. -!- Dehydrogenation forms an imine, which dissociates to the amino acid and pyruvate. -!- In the reverse direction, the enzyme acts also on neutral amino acids as an amino donor. -!- They include L-amino acids such as 2-aminopentanoic acid, 2-aminobutyric acid, 2-aminohexanoic acid, 3-chloroalanine, O-acetylserine, methionine, isoleucine, valine, phenylalanine, leucine and alanine. -!- The amino acceptors include 2-oxoacids such as pyruvate, oxaloacetate, glyoxylate and 2-oxobutyrate.
|
UniProtKB Entries (1)
| Q44297 |
ODH_ARTSC
Arthrobacter sp. 1C
Opine dehydrogenase
|
PDB Structure
| PDB | 1BG6 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Crystal structure and active site location of N-(1-D-carboxylethyl)-L-norvaline dehydrogenase.
Nat.Struct.Biol.
|