This superfamily entry represents the thioesterase domain with a hot-dog fold of Rv0098. According to Pfam, this family was formerly known as DUF2662. Rv0098 is part of an operon, Rv0096-Rv0101, from Mycobacterium tuberculosis (Mtb) that is essential for Mtb's survival in mouse macrophages. This operon also contains an acyl carrier protein and one of the only two nonribosomal peptide synthases in Mtb. Rv0098 was proposed to have a fatty acyl-CoA thioesterase (FcoT) function, with preference for long chain fatty acyl groups PMID:17524985. The maximal Kcat was observed for palmitoyl-CoA, although longer and shorter molecules were also cleaved. In solution this protein forms a homo-hexameric complex.

The Mtb FcoT protomer has an alpha/beta "hot dog" fold, in which an antiparallel beta sheet (B1-B6), referred to in the literature as the "bun," wraps around a six-turn alpha helix (H3) typically called the "sausage." Three short alpha helices (H1, H2, and H4) cover the open side of the "bun". This "hot dog" topology is common for dehydratases and thioesterases.

PFAM:PF10862, INTERPRO:IPR022598,DOI:10.1016/j.chembiol.2007.04.005